Zeiss AxioPlan 2i Upright Fluorescent Microscope
- Extensively used in facility for brightfield, DIC, and fluorescent microscopy
- Uses a broad spectrum EFOS Xcite50 lightsource for fluorescent imaging
- Objectives:
- 2.5x dry (0.075 NA)
- 5x dry (0.15 NA)
- 10x dry (0.3 NA)
- 20x dry (0.5 NA)
- 40x dry (0.75 NA)
- 63x oil (1.4 NA)
- 100x oil (1.4 NA)
- Fluorescence imaging filter sets:
- DAPI
- GFP/FITC
- Rhodamine/Texas Red
- BlueFP
- Pinkel40
- EndowGFP
- External excitation and emmision filters allow for fast response dual excitation
- Fully automated stage, objectives, filter sets and general operations via a computer control console
- Integrated halogen light source allows for brightfield and DIC imaging
- Image acquisition is performed via the AxioCam HRm (monochrome), the Hammamatsu Orca (high-res, low noise monochrome), or the AxioCam MRc color camera
- Image analysis and processing is handled via the Zeiss AxioVision software
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