Protocols

Protocols

For a description of the protocols used in the Imaging Facility Wet Lab, click on any of the links below.All protocols will open in a new window in a Adobe Acrobat’s PDF format for printing. To get the Acrobat PDF plug-in, click here.

Brightfield Protocols

• H&E Staining
• Giemsa
• Periodic Acid Schiff (PAS)
• Toluidine Blue
• Cryoembedding Protocol

Fluorescent Protocols

• Primary Conjugate Staining
• Secondary Conjugate Staining
• DAPI
• Cryoembedding Protocol

TEM Protocols

• Lead Staining
• Negative Staining
• Immunogold Labeling
• Darkroom Procedures

Microscope Equipment

• Manual Optics Cleaning