Hitachi H-7600 TEM — The facility is equipped with a JEOL JEM-1200EX (120,000 volt) transmission electron microscope for viewing thin sections or negatively-stained/shadowed particles. Magnifications of X50 to X1,000,000 with 1.4-Angstrom resolution can be achieved with this microscope. A high-resolution digital CCD camera system and associated acquisition computer are attached to the microscope for instant capture of EM micrographs. In addition, images can still be captured on negatives, which will be processed by the Imaging staff
Zeiss LSM510 Confocal Microscope — A Zeiss LSM 510 laser scanning microscope with a UV laser and META attachment for spectral imaging. This microscope provides state-of-the-art confocal imaging of the entire range of fluorochromes currently in use. The confocal stand is an inverted (Zeiss Axiovert 2IE) scope making it particularly useful for live cell work.
Zeiss AxioPlan 2i — This microscope is equipped with an optical fiber-coupled XCite illuminator (EFOS) for increased uniformity and stability of illumination intensity as well as the new Zeiss harmonic drive for accurate focus changes. An above-binocular filter wheel allows certain dual-wavelength acquisitions with zero image shift between wavelengths (for example, CFP/YFP). This scope is equipped with a Solamere XR-Mega12 Gen IV intensified CCD camera for rapid acquisition of low-light images. Automated stage, focusing and filter control allow 4D microscopy at multiple wavelengths and at several areas of a single preparation, unattended. Deconvolution routines are available using Autodeblur (AutoQuant, Inc.). Zeiss KS400 and the recently upgraded Axiovision (Zeiss)are available to facilitate image processing and analysis.
Zeiss Axiovert 200m — A Leica CM3050 research cryostat (provided by Dr. Paul Kincade – OMRF) is housed in the Imaging Facility to obtain frozen sections (2-60 mm) of fixed or unfixed tissues. Sample preparation for the cryostat includes freezing and embedding fixed or fresh tissues in the freezing medium. Trained users are allowed to operate the cryostat, or the Imaging staff can obtain cryosections for untrained users.
Olympus Dissection —