Imaging

OMRF’s Imaging Core Facility assists researchers with imaging needs ranging from basic histology, immunofluorescence and electron microscopy to digital image processing and analysis. Funding for the facility comes from OMRF, NIH and recharges to users.

Our facility personnel serve as technical consultants, train new users, advance new imaging technologies and provide specialized imaging services in cooperation with OMRF and OUHSC researchers.

To arrange a consultation or a tour of the facility, contact:

Ben Fowler, Imaging Facility Manager

Phone: (405) 271-7245 or (405) 271-7545

Email:  fowlerb@omrf.org

MAP

OMRF Imaging Core uses iLabs for scheduling. Please use the following links to login to iLabs to schedule equipment.

OMRF

OUHSC

Other Organizations

If you don not have a iLabs login or are not sure please contact the Imaging Core at 405-271-7545

Facility Equipment

From basic histology to advanced laser scanning confocal microscopy, we have a wide variety of equipment to facilitate our users’ needs. Below are links to out equipment including descriptions and uses.

Sample Preparation

Microscopy

Computers and Printers

OMRF Pricing

External Educational Pricing

Corporate Pricing

For Imaging Core Charges also check iLabs, contact the department at 405-271-7545, or email Ben-Fowler@omrf.org.

Protocols

For a description of the protocols used in the Imaging Facility Wet Lab, click on any of the links below.All protocols will open in a new window in a Adobe Acrobat’s PDF format for printing. To get the Acrobat PDF plug-in, click here.

Brightfield Protocols

• H&E Staining
• Giemsa
• Periodic Acid Schiff (PAS)
• Toluidine Blue
• Cryoembedding Protocol

Fluorescent Protocols

• Primary Conjugate Staining
• Secondary Conjugate Staining
• DAPI
• Cryoembedding Protocol

TEM Protocols

• Lead Staining
• Negative Staining
• Immunogold Labeling
• Darkroom Procedures

Microscope Equipment

• Manual Optics Cleaning

Histology

• Leica
• ThermoShandon
• Balzers
• Denton
• EMS/Diatome
• Fisher Scientific
• VWR
• Molecular Probes
• Jackson Immuno Research
• Vector Labs
• BIO

Microscopy

• Zeiss
• Hitachi
• Olympus
• Nikon
• Solamere
• Canon
• Fluorochrome Spectra Catalog – see spectral signatures for different fluorochromes
• Axioplan and FLuorescence Tutorial
• Becker & Hickl GmbH ‘The bh TCSPC Handbook’.

Software Downloads

• Zeiss AxioVision LE – For Axioplan and Axiovert Microscopes
• Zeiss AIM Image Examiner – for the LSM-510META
• Zeiss AIM Image Browser 4.0 – for the LSM-510META
• Becker & Hickl GmbH TCSPC software for 32bit Windows
• Becker & Hickl GmbH TCSPC software for 64bit Windows

Other

• Zcorp
• Fujifilm
• TeraRecon
• Kitware
• Autoquant

Microscopy Societies

• Microscopy Society of America
• Microscopy Society of Oklahoma
• Microscopy and Microanalysis Conference