Zeiss LSM-510META Laser Scanning Confocal Microscope
- Used for single and muliple fluorphores, capable of image acquisition in the X, Y, and Z directions, and for optical serial sectioning in the z-direction (stacks for volumetric analysis)
- Several stage inserts are available that facilitate the viewing of slides and culture dishes, as well as a temperature controlled chamber
- META Spectral Detector allows separation of fluorphores with significant spectral overlap (ie GFP and FITC)
- Objectives:
- 10x dry (0.3 NA)
- 25x water (0.7 NA)
- 40x multi-immersion (1.2 NA)
- 63x water (1.2 NA)
- 63x oil (1.4 NA)
- 100x oil (1.4 NA)
- Laser Excitation and common fluorophores:
- Enterprise:
- 364nm – DAPI and Hoescht
- Argon:
- 458nm – CFP
- 514nm – YFP
- 488nm – FITC, Cy2, GFP/eGFP, Alexa488
- HeNe1:
- 543nm – Rhodamine, Alexa546, Cy3
- HeNe2:
- 633nm – Cy5, PE
- Enterprise:
- Users must be trained by Imaging staff and approved for use
- Click here to check the calendar for availability or to make a reservation