OMRF’s Imaging Core Facility assists researchers with imaging needs ranging from basic histology, immunofluorescence and electron microscopy to digital image processing and analysis. Funding for the facility comes from OMRF, NIH and recharges to users.
Our facility personnel serve as technical consultants, train new users, advance new imaging technologies and provide specialized imaging services in cooperation with OMRF and OUHSC researchers.
To arrange a consultation or a tour of the facility, contact:
Ben Fowler, Imaging Facility Manager
Phone: (405) 271-7245 or (405) 271-7545
OMRF Academic Pricing
Histological Preparation and Equipment - More Info
|Tissue Processing||1-30 Cassettes – $6.00/cassette
31+ cassettes – $5.00/cassette
|Slides (H&E stained) – includes coverslipping||$8.00/rack|
- Tissue sections from paraffin microtome on appropriate slides. Each slide typically holds 3-6 sections.
- A rack carries up to 19 slides. H&E staining and coverslipping takes ~1.5 hours.
- Frozen tissue sections obtained from Leica CM3050 cryostat. One slide typically holds 3 sections.
EM Sample Preparation and Equipment - More Info
|High Pressure Freezing||$100.00 setup + $20.00/hour|
|Cryoultramicrotome||$40.00 setup + $25.00/hour|
|Heavy Metal Staining||(up to 10 grids) $12.00/run|
- Fixatives (aldehydes, OsO4), dehydrants and resins included in the cost. Processing time is 1-2 weeks.
- Diamond knife charges offset resharpening costs.
- Gold-labeled secondary antibodies supplied (see above) and heavy metal staining
Microscopy Equipment - More Info
|Hitachi H7600 Transmission Electron Microscope||$35.00/hour|
|Zeiss LSM 510 or 710 Laser Scanning Confocal Microscope||$30.00/hour|
|Zeiss Axioplan 2i Upright Microscope||$15.00/hour|
|Zeiss Axiovert 200m Inverted Microscope||$15.00/hour|
|Olympus Dissection Microscope||$7.50/hour|
|Zeiss Axiostar Brightfield Microscope||$7.00/hour|
|TIRF Microscope||$30/1st hour; $20/each additional hour|
|EM Negatives||$15.00 setup + $1.00/negative|
Computers and Printers - More Info
|3d Imaging and Deconvolution Workstation||$7.00/hour|
|Prints – Fujix Pictography photo printer||$7.00 full/$3.00 half|
|Prints – Olympus Dye Sub (4″ x 5″)||$1.00/print|
Imaging Staff Labor Charges
|General Labor (Preparation)||$20/hour|
|Specialized Technical Assistance (includes Imaging)||$30/hour|
Imaging Facility Supplies
|Grid storage box||$18.50|
|Slide Box – cardboard (20 slides)||$15.00|
|Slide Box – plastic (25 slides)||$8.50|
|Slide Box – plastic (100 slides)||$12.00|
|Gelatin subbed slides||$1.00/slide|
|Superfrost charges slides||$30.00/box or $0.50/slide|
|For other items, please inquire in the Facility|
Prices effective July 1, 2011, subject to change without notice
For a description of the protocols used in the Imaging Facility Wet Lab, click on any of the links below.All protocols will open in a new window in a Adobe Acrobat’s PDF format for printing. To get the Acrobat PDF plug-in, click here.